Plasmid

Part:BBa_J100434:Design

Designed by: Morgan Klein   Group: Campbell M Lab   (2018-07-03)


pClone mScarlet with Plac Control Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1094
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1094
    Illegal BamHI site found at 1040
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1094
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1094
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 753
    Illegal BsaI.rc site found at 969


Design Notes

Built PCR primers to amplify only the plac promoter region of J04450 and added BsaI sites and the necessary pClone mScarlet sticky ends.


Source

Comes from the pClone mScarlet plasmid and the J04450 plasmid.

References